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Food Tests Aim To use qualitative reagents to test for a range of carbohydrates lipids and proteins To include Benedict’s test for sugars Iodine test for starch the emulsion test for lipids and the Biuret reagent for protein You will Use qualitative reagents to test for the presence of key biological molecules in a range of foods.
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For a solid sample prepare a test solution by crushing the food in a mortar and pestle and adding a moderate amount of distilled water Decant the suspension to remove large particles Use the decanted liquid as the test solution Add 2 cm 3 of the sample solution to a test tube Add 1 cm 3 of dilute hydrochloric acid and boil for one minute.
Testing for Starch, Sugar and Protein in Foods
CIE A level biology notes Ethanol emulsion test sample has to be crushed add alcohol to it to make a solution add water shake the tube if the cloudy emulsion forms then it shows presence of lipids.
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You will be using these tests on a variety of food substances Tests to be demonstrated Biuret test for Proteins Add Biuret solution (sodium hydroxide + small amount of copper sulphate) blue like Benedict’s to the test substance THERE IS NO NEED TO HEAT but the result is not quite instantaneous.
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There are different tests which can be used to detect carbohydrates proteins and lipids They involve adding a reagent to a food sample which changes.